Wee1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # H00007465-D01P
Key Product Details
Species Reactivity
Human
Applications
Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
WEE1 (AAH51831.1, 1 a.a. - 646 a.a.) full-length human protein. MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYRNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY
Specificity
WEE1 - WEE1 homolog (S. pombe),
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Scientific Data Images for Wee1 Antibody
![Western Blot: Wee1 Antibody [H00007465-D01P]](https://resources.bio-techne.com/images/products/Wee1-Antibody-Western-Blot-H00007465-D01P-img0003.jpg "Western Blot: Wee1 Antibody [H00007465-D01P]")
Western Blot: Wee1 Antibody [H00007465-D01P]
Western Blot: Wee1 Antibody [H00007465-D01P] - Analysis of WEE1 expression in transfected 293T cell line by WEE1 polyclonal antibody.Lane 1: WEE1 transfected lysate(71.70 KDa).Lane 2: Non-transfected lysate.![Proximity Ligation Assay: Wee1 Antibody [H00007465-D01P]](https://resources.bio-techne.com/images/products/Wee1-Antibody-Proximity-Ligation-Assay-H00007465-D01P-img0002.jpg "Proximity Ligation Assay: Wee1 Antibody [H00007465-D01P]")
Proximity Ligation Assay: Wee1 Antibody [H00007465-D01P]
Proximity Ligation Assay: Wee1 Antibody [H00007465-D01P] - Analysis of protein-protein interactions between WEE1 and FBXW11. HeLa cells were stained with anti-WEE1 rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Applications for Wee1 Antibody
Application
Recommended Usage
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Wee1
Long Name
Wee1-like protein kinase
Alternate Names
Wee1A kinase, WEE1hu
Entrez Gene IDs
7465 (Human)
Gene Symbol
WEE1
UniProt
Additional Wee1 Products
Product Documents for Wee1 Antibody
Product Specific Notices for Wee1 Antibody
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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