WIPF1/WIP Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15508

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WIPF1/WIP Antibodies »

Product Summary for WIPF1/WIP Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human WIPF1/WIP (NP_001070737.1). QLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAPPLPP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WIPF1/WIP Antibody - Azide and BSA Free

Western Blot: WIPF1/WIP AntibodyAzide and BSA Free [NBP3-15508]

Western Blot: WIPF1/WIP AntibodyAzide and BSA Free [NBP3-15508]

Western Blot: WIPF1/WIP Antibody [NBP3-15508] - Western blot analysis of extracts of various cell lines, using WIPF1/WIP Rabbit pAb (NBP3-15508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - Azide and BSA Free [NBP3-15508]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - Azide and BSA Free [NBP3-15508]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody [NBP3-15508] - Immunohistochemistry of paraffin-embedded mouse spleen using WIPF1/WIP antibody (NBP3-15508) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - Azide and BSA Free [NBP3-15508]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - Azide and BSA Free [NBP3-15508]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody [NBP3-15508] - Immunohistochemistry of paraffin-embedded human appendix using WIPF1/WIP antibody (NBP3-15508) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for WIPF1/WIP Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WIPF1/WIP

WIPF1 is involved in the organization of the actin cytoskeleton. The protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these WIPF1 and Wiskott-Aldrich syndrome protein may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. WIPF1 binds to Wiskott-Aldrich syndrome protein, profilin and actin. It is involved with NCK1 and GRB2 in the recruitment and activation of N-WASP and may be involved in regulating the subcellular localization of N-WASP, resulting in the disassembly of stress fibers in filopodia formation.

Alternate Names

WAS/WASL interacting protein family, member 1, WAS/WASL-interacting protein family member 1, WASP-interacting protein, WASPIP, WIPMGC111041, WiskProtein PRPL-2, WiskPRPL-2

Gene Symbol

WIPF1

Additional WIPF1/WIP Products

Product Documents for WIPF1/WIP Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WIPF1/WIP Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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