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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

WIPI1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WIPI1. The immunogen is located within amino acids 350 - 400 of WIPI1. Amino Acid Squence: LGSGTTEENKENDLRPS

Specificity

At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WIPI1 Antibody - BSA Free

Western Blot: WIPI1 AntibodyBSA Free [NBP2-82048]

Western Blot: WIPI1 AntibodyBSA Free [NBP2-82048]

Western Blot: WIPI1 Antibody [NBP2-82048] - Analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 ug/ml.
WIPI1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: WIPI1 Antibody - BSA Free [NBP2-82048] -

Immunocytochemistry/ Immunofluorescence: WIPI1 Antibody - BSA Free [NBP2-82048] - Immunofluorescence of WIPI1 in human colon tissue with WIPI1 antibody at 20 ug/mL.

Applications for WIPI1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WIPI1

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Alternate Names

Atg18, Atg18 protein homolog, ATG18A, FLJ10055, WD repeat domain phosphoinositide-interacting protein 1, WD repeat domain, phosphoinositide interacting 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WD40 repeat protein Interacting with phosphoInositides of 49kDa, WIPI 49 kDa, WIPI-1, WIPI-1 alpha, WIPI49ATG18

Gene Symbol

WIPI1

Additional WIPI1 Products

Product Documents for WIPI1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WIPI1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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