WIRE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86858

Novus Biologicals, part of Bio-Techne
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NBP1-86858
NBP1-86858-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WIRE Antibodies »

Product Summary for WIRE Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PVNIRTGPSGQSLAPPPPPYRQPPGVPNGPSSPTNESAPELPQRHNSLHRKTPGPVRGLAPPPPTSASPSLLSNRPPP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WIRE Antibody

Western Blot: WIRE Antibody [NBP1-86858]

Western Blot: WIRE Antibody [NBP1-86858]

Western Blot: WIRE Antibody [NBP1-86858] - Analysis using Anti-WIPF2 antibody NBP1-86858 (A) shows similar pattern to independent antibody NBP1-86857 (B).
Immunocytochemistry/ Immunofluorescence: WIRE Antibody [NBP1-86858]

Immunocytochemistry/ Immunofluorescence: WIRE Antibody [NBP1-86858]

Immunocytochemistry/Immunofluorescence: WIRE Antibody [NBP1-86858] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86858]

Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86858]

Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86858] - Analysis in human cerebral cortex and liver tissues using NBP1-86858 antibody. Corresponding WIRE RNA-seq data are presented for the same tissues.
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Applications for WIRE Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WIRE

Alternate Names

PP10631, WAS/WASL interacting protein family, member 2, WIP-related protein

Gene Symbol

WIPF2

Additional WIRE Products

Product Documents for WIRE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WIRE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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