WISP-1/CCN4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77339

Novus Biologicals, part of Bio-Techne
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NB100-77339
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all WISP-1/CCN4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ESYPDFSEIAN corresponding to C-Terminus according to NP_543028.1.

Epitope

ESYPDFSEIAN

Specificity

This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WISP-1/CCN4 Antibody

Western Blot: WISP-1/CCN4 Antibody [NB100-77339]

Western Blot: WISP-1/CCN4 Antibody [NB100-77339]

Western Blot: WISP-1/CCN4 Antibody [NB100-77339] - Staining of Caco-2 cell lysate 1 (A) and Caco-2 cell lysate 2 (B) + petide (C) with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: WISP-1/CCN4 Antibody [NB100-77339]

Immunocytochemistry/ Immunofluorescence: WISP-1/CCN4 Antibody [NB100-77339]

Immunocytochemistry/Immunofluorescence: WISP-1/CCN4 Antibody [NB100-77339] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: WISP-1/CCN4 Antibody [NB100-77339]

Flow Cytometry: WISP-1/CCN4 Antibody [NB100-77339]

Flow Cytometry: WISP-1/CCN4 Antibody [NB100-77339] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for WISP-1/CCN4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated MW of 30.7 kDa according to NP_543028.1 and 40.3 kDa according to NP_003873.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WISP-1/CCN4

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants.

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Entrez Gene IDs

8840 (Human); 22402 (Mouse); 65154 (Rat)

Gene Symbol

CCN4

UniProt

O95388

Additional WISP-1/CCN4 Products

Product Documents for WISP-1/CCN4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for WISP-1/CCN4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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