WNK1 Antibody (HL2184) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25748
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2184
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragmemt of human WNK1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for WNK1 Antibody (HL2184) - Azide and BSA Free
Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:500. Blue: Fluoroshield with DAPI .
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .
Applications for WNK1 Antibody (HL2184) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: WNK1
WNK1 mutations have been associated with Gordon hyperkalemia-hypertension syndrome (pseudohypoaldosteronism type II) and congenital sensory neuropathy.
WNK1 is also known as Human Accelerated Region 5, and is thought to have played a key role in differentiating humans from apes.
Long Name
WNK Lysine-deficient Protein Kinase 1
Alternate Names
KDP, PHA2C, PRKWNK1
Gene Symbol
WNK1
Additional WNK1 Products
Product Documents for WNK1 Antibody (HL2184) - Azide and BSA Free
Product Specific Notices for WNK1 Antibody (HL2184) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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