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WNK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33875

Novus Biologicals, part of Bio-Techne
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NBP2-33875
NBP2-33875-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASST

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WNK2 Antibody

Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875]

Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875]

Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] - Staining of human cerebral cortex, colon, fallopian tube and liver using Anti-WNK2 antibody NBP2-33875 (A) shows similar protein distribution across tissues to independent antibody NBP2-33876 (B).
Immunocytochemistry/ Immunofluorescence: WNK2 Antibody [NBP2-33875]

Immunocytochemistry/ Immunofluorescence: WNK2 Antibody [NBP2-33875]

Immunocytochemistry/Immunofluorescence: WNK2 Antibody [NBP2-33875] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875]

Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875]

Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] - Staining of human liver using Anti-WNK2 antibody NBP2-33875.

Applications for WNK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WNK2

WNK2 is encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase activity, it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved lysine.

Alternate Names

EC 2.7.11, EC 2.7.11.1, PRK, PRKWNK2, Protein kinase with no lysine 2, SDCCAG43, serine/threonine-protein kinase WNK2, Serologically defined colon cancer antigen 43lysine deficient 2, WNK lysine deficient protein kinase 2

Gene Symbol

WNK2

UniProt

Additional WNK2 Products

Product Documents for WNK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WNK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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