Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 13F9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Wnt-1 Antibodies »

Product Summary for Wnt-1 Antibody (13F9)

Immunogen

Synthetic peptide corresponding to an internal region of human Wnt-1 protein. (Uniprot: P04628)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Wnt-1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt-1 based on a 91% sequence homology. Cross-reactivity with Wnt-1 from other sources has not been determined.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Wnt-1 Antibody (13F9)

Western Blot: Wnt-1 Antibody (13F9) [NBP1-42739]

Western Blot: Wnt-1 Antibody (13F9) [NBP1-42739]

Western Blot: Wnt-1 Antibody (13F9) [NBP1-42739] - Analysis of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the 41 kDa Wnt1 protein. Primary antibody was used at a 1:500 dilution.
Wnt-1 Antibody (13F9)

Wnt-1 Antibody (13F9)

Western blot using Protein A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kDa Wnt1 protein. Primary antibody was used at a 1:500 dilution. Personal communication, Stephen Brown, Brown University

Applications for Wnt-1 Antibody (13F9)

Application
Recommended Usage

ELISA

1:4000-1:20000

Western Blot

1:175-1:250
Application Notes
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Wnt-1

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Alternate Names

Int-1, Wnt1

Entrez Gene IDs

7471 (Human)

Gene Symbol

WNT1

UniProt

P04628 (Human)

Additional Wnt-1 Products

Product Documents for Wnt-1 Antibody (13F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-1 Antibody (13F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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