Wnt-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77948
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Wnt-1 Antibody
Immunogen
This affinity purified Wnt-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt-1 protein. (Uniprot: P04628)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with Wnt-1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt-1 based on a 91% sequence homology. Cross-reactivity with Wnt-1 from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Wnt-1 Antibody
Western Blot: Wnt-1 Antibody [NBP1-77948]
Western Blot: Wnt-1 Antibody [NBP1-77948] - This antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt-1 (data not shown).Applications for Wnt-1 Antibody
Application
Recommended Usage
ELISA
1:25000
Western Blot
1:1500-1:6000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Wnt-1
Long Name
Wingless-type MMTV Integration Site Family, Member 1
Alternate Names
Int-1, Wnt1
Gene Symbol
WNT1
UniProt
Additional Wnt-1 Products
Product Documents for Wnt-1 Antibody
Product Specific Notices for Wnt-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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