Wnt-3a Antibody (1E6G4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52588

Novus Biologicals, part of Bio-Techne
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NBP2-52588
NBP2-52588-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1E6G4

Format

BSA Free

Concentration

1 mg/ml

View all Wnt-3a Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Wnt-3a (AA: 170-352) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-3a Antibody (1E6G4) - BSA Free

Western Blot: Wnt-3a Antibody (1E6G4)BSA Free [NBP2-52588]

Western Blot: Wnt-3a Antibody (1E6G4)BSA Free [NBP2-52588]

Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mouse mAb against A549 (1), HepG2 (2), and A431 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Wnt-3a Antibody (1E6G4) - BSA Free [NBP2-52588]

Immunocytochemistry/ Immunofluorescence: Wnt-3a Antibody (1E6G4) - BSA Free [NBP2-52588]

Immunocytochemistry/Immunofluorescence: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of HeLa cells using WNT3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) - BSA Free [NBP2-52588]

Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) - BSA Free [NBP2-52588]

Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of endometrial cancer tissues, mouse mab with DAB staining.
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Applications for Wnt-3a Antibody (1E6G4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-3a

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Long Name

Wingless-type MMTV Integration Site Family, Member 3a

Alternate Names

Wnt3a

Gene Symbol

WNT3A

Additional Wnt-3a Products

Product Documents for Wnt-3a Antibody (1E6G4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-3a Antibody (1E6G4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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