Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A6

Format

BSA Free

Concentration

1.0 mg/ml

View all Wnt-3a Antibodies »

Product Specifications

Immunogen

Recombinant human Wnt-3a (19-352aa) purified from E. coli (NP_149122).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Wnt-3a Antibody (3A6) - BSA Free

Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]

Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]

Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]

Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]

Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]

Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]

Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for Wnt-3a Antibody (3A6) - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:100SDS-PAGE 1:100-1:2000

Western Blot

1:1000-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26708597)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-3a

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Long Name

Wingless-type MMTV Integration Site Family, Member 3a

Alternate Names

Wnt3a

Entrez Gene IDs

89780 (Human)

Gene Symbol

WNT3A

UniProt

P56704 (Human)

Additional Wnt-3a Products

Product Documents for Wnt-3a Antibody (3A6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-3a Antibody (3A6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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