Wnt-3a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-79724

Novus Biologicals, part of Bio-Techne
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NBP1-79724
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Wnt-3a Antibodies »

Product Specifications

Immunogen

Synthetic peptide directed towards the N terminal of human WNT3AThe immunogen for this antibody is WNT3A (NP_149122). Peptide sequence MAPLGYFLLLCSLKQALGSYPIWWSLAVGPQYSSLGSQPILCASIPGLVP. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Wnt-3a Antibody

Western Blot: Wnt-3a Antibody [NBP1-79724]

Western Blot: Wnt-3a Antibody [NBP1-79724]

Western Blot: Wnt-3a Antibody [NBP1-79724] - Titration: 0.2-1 ug/ml, Positive Control: Human heart.
Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-79724]

Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-79724]

Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-79724] - Human prostate tissue at an antibody concentration of 2.5 ug/ml.

Applications for Wnt-3a Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-3a

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Long Name

Wingless-type MMTV Integration Site Family, Member 3a

Alternate Names

Wnt3a

Entrez Gene IDs

89780 (Human)

Gene Symbol

WNT3A

UniProt

P56704 (Human)

Additional Wnt-3a Products

Product Documents for Wnt-3a Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-3a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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