Wnt-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1006
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for Wnt-4 Antibody
Immunogen
Peptide with sequence C-SNWLYLAKLSSVGS corresponding to internal region according to NP_110388.2.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat.
Localization
Possibly secreted and associates with the extracellular matrix.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Wnt-4 Antibody
![Western Blot: Wnt-4 Antibody [NB100-1006]](https://resources.bio-techne.com/images/products/Wnt-4-Antibody-Western-Blot-NB100-1006-img0002.jpg "Western Blot: Wnt-4 Antibody [NB100-1006]")
Western Blot: Wnt-4 Antibody [NB100-1006]
Western Blot: Wnt-4 Antibody [NB100-1006] - (1ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]](https://resources.bio-techne.com/images/products/Wnt-4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1006-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]")
Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]
Immunocytochemistry/Immunofluorescence: Wnt-4 Antibody [NB100-1006] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue)Applications for Wnt-4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:4000
Western Blot
1:100-1:2000
Application Notes
WB: Approx. 40+35 kDa band observed in Jurkat lysates (calculated MW of 39.1 kDa band according to NP_110388.2 Negative Control: U937 cell lysate.).
Immunofluorescence: Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells.
Immunofluorescence: Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Wnt-4
Long Name
Wingless-type MMTV Integration Site Family, Member 4
Alternate Names
Wnt4
Gene Symbol
WNT4
UniProt
Additional Wnt-4 Products
Product Documents for Wnt-4 Antibody
Product Specific Notices for Wnt-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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