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Wnt-5a Antibody (S08-4A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20061

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-20061-100ul
NBP3-20061-50ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-4A3

Concentration

0.3 mg/ml

Product Specifications

Immunogen

A synthetic peptide of human Wnt-5a (Uniprot # P41221)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-5a Antibody (S08-4A3)

Western Blot: Wnt-5a Antibody (S08-4A3) [NBP3-20061]

Western Blot: Wnt-5a Antibody (S08-4A3) [NBP3-20061]

Western Blot: Wnt-5a Antibody (S08-4A3) [NBP3-20061] - Western blot detection of Wnt-5a in Hela cell lysates using NBP3-20061 (1:500 diluted). Predicted band size: 42kDa. Observed band size: 45kDa.
Immunocytochemistry/ Immunofluorescence: Wnt-5a Antibody (S08-4A3) [NBP3-20061]

Immunocytochemistry/ Immunofluorescence: Wnt-5a Antibody (S08-4A3) [NBP3-20061]

Immunocytochemistry/Immunofluorescence: Wnt-5a Antibody (S08-4A3) [NBP3-20061] - Immunocytochemistry of Wnt-5a (green) in hela using NBP3-20061 at dilution 1/50, and DAPI (blue)

Applications for Wnt-5a Antibody (S08-4A3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Gene Symbol

WNT5A

Additional Wnt-5a Products

Product Documents for Wnt-5a Antibody (S08-4A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-5a Antibody (S08-4A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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