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Wnt-7b Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59564

Novus Biologicals, part of Bio-Techne
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NBP1-59564

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to WNT7B(wingless-type MMTV integration site family, member 7B) The peptide sequence was selected from the middle region of WNT7B (NP_478679) Peptide sequence WTTLPKFREVGHLLKEKYNAAVQVEVVRASRLRQPTFLRIKQLRSYQKPM. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29871934)..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Wnt-7b Antibody

Western Blot: Wnt-7b Antibody [NBP1-59564]

Western Blot: Wnt-7b Antibody [NBP1-59564]

Western Blot: Wnt-7b Antibody [NBP1-59564] - Titration: 0.2-1 ug/ml, Positive Control: Human kidney.
Immunohistochemistry-Paraffin: Wnt-7b Antibody [NBP1-59564]

Immunohistochemistry-Paraffin: Wnt-7b Antibody [NBP1-59564]

Immunohistochemistry-Paraffin: Wnt-7b Antibody [NBP1-59564] - Human Brain, cortex tissue at an antibody concentration of 4-8ug/ml.

Applications for Wnt-7b Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID25823570)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-7b

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fat

Long Name

Wingless-type MMTV Integration Site Family, Member 7B

Alternate Names

Wnt7b

Entrez Gene IDs

7477 (Human)

Gene Symbol

WNT7B

UniProt

Additional Wnt-7b Products

Product Documents for Wnt-7b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-7b Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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