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Wnt-7b Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93321

Novus Biologicals, part of Bio-Techne
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NBP2-93321-0.02ml
NBP2-93321-0.1ml

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Wnt-7b Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human WNT7B (NP_478679.1). TAACSQGNLSNCGCDREKQGYYNQAEGWKWGGCSADVRYGIDFSRRFVDAREIKKNARRLMNLHNNEAGRKVLEDRMQLECKCHGVSGSCTTKTCWTTLPKFREVGHLLKEKYNAAVQVEVVRASRLRQPTFLRIKQLRSYQKPMETDLVYIEKSPNYCEEDAATGSVGTQGRLCNRTSPGADGCDTMCCGRGYNTHQYTKVWQCNCKFHWCCFVKCNTCSERTEVFTCK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-7b Antibody - BSA Free

Western Blot: Wnt-7b AntibodyBSA Free [NBP2-93321]

Western Blot: Wnt-7b AntibodyBSA Free [NBP2-93321]

Western Blot: Wnt-7b Antibody [NBP2-93321] - Analysis of extracts of various cell lines, using Wnt-7b at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Applications for Wnt-7b Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Wnt-7b

Wnt7b is a gene that codes for a protein that is 349 amino acids in length and approximately 39 kDa in weight that is a ligand for members of the frizzled family of transmembrane receptors that may function to facilitate the development of discrete region of tissues. Current studies are being done on several diseases and disorders linked to this gene including carcinoma, leukemia, fainting, hermaphroditism, melanoma, prostatitis, neuronitis, and colorectal cancer. Wnt7b has also been shown to have interactions with FZD1, FZD10, FZD5, GPC3, and LRP5 in pathways such as the GSK3 signaling, colorectal cancer metastasis, DNA damage response, and GPCR ligand binding pathways.

Long Name

Wingless-type MMTV Integration Site Family, Member 7B

Alternate Names

Wnt7b

Gene Symbol

WNT7B

Additional Wnt-7b Products

Product Documents for Wnt-7b Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-7b Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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