Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Wnt-3 Antibodies »

Product Summary for Wnt3 Antibody

Immunogen

Synthetic peptide from the C-terminal of Human Proto-oncogene Wnt-3 (aa. 280-330)

Localization

Secreted|Extracellular Space|Extracellular Matrix

Specificity

50 kDa band is due to post translational modifications.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt3 Antibody

Western Blot: Wnt3 Antibody [NBP3-18245]

Western Blot: Wnt3 Antibody [NBP3-18245]

Western Blot: Wnt3 Antibody [NBP3-18245] - Western blot analysis of Mouse Brain showing detection of 39.6 kDa Wnt3 protein using Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 39.6 kDa. Other Band(s): 50 kDa.
Immunohistochemistry: Wnt3 Antibody [NBP3-18245]

Immunohistochemistry: Wnt3 Antibody [NBP3-18245]

Immunohistochemistry: Wnt3 Antibody [NBP3-18245] - Immunohistochemistry analysis using Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Applications for Wnt3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS pH 7.4, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Wnt-3

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.

Long Name

Wingless-type MMTV Integration Site Family, Member 3

Alternate Names

INT4, TETAMS, WNT3

Gene Symbol

WNT3

Additional Wnt-3 Products

Product Documents for Wnt3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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