WSTF Antibody (5E9)
Novus Biologicals, part of Bio-Techne | Catalog # H00009031-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5E9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BAZ1B (NP_075381, 1384 a.a. ~ 1483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK
Localization
Nuclear
Specificity
BAZ1B - bromodomain adjacent to zinc finger domain, 1B
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for WSTF Antibody (5E9)
![Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]](https://resources.bio-techne.com/images/products/WSTF-Antibody-5E9-Immunohistochemistry-Paraffin-H00009031-M01-img0005.jpg "Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]")
Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]
Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01] - Analysis of monoclonal antibody to BAZ1B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.![Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]](https://resources.bio-techne.com/images/products/WSTF-Antibody-5E9-Proximity-Ligation-Assay-H00009031-M01-img0002.jpg "Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]")
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. HeLa cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).![Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]](https://resources.bio-techne.com/images/products/WSTF-Antibody-5E9-Proximity-Ligation-Assay-H00009031-M01-img0003.jpg "Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]")
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. Huh7 cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Applications for WSTF Antibody (5E9)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. It has been used for IHC-P.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: WSTF
Alternate Names
bromodomain adjacent to zinc finger domain, 1B, williams-Beuren syndrome chromosomal region 10 protein
Entrez Gene IDs
9031 (Human)
Gene Symbol
BAZ1B
OMIM
605681 (Human)
Additional WSTF Products
Product Documents for WSTF Antibody (5E9)
Product Specific Notices for WSTF Antibody (5E9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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