WT1 Antibody (6L9X6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16135
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6L9X6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544). PPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for WT1 Antibody (6L9X6)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135]](https://resources.bio-techne.com/images/products/WT1-Antibody-6L9X6-Western-Blot-NBP3-16135-img0006.jpg "Western Blot: WT1 Antibody (6L9X6) [NBP3-16135]")
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135]
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] -
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of lysates from Mouse testis using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] -
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of various lysates using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for WT1 Antibody (6L9X6)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: WT1
Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.
Long Name
Wilms Tumor 1
Alternate Names
GUD, WAGR, WIT-2, WT33
Gene Symbol
WT1
Additional WT1 Products
Product Documents for WT1 Antibody (6L9X6)
Product Specific Notices for WT1 Antibody (6L9X6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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