WT1 Antibody (CL13165)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18564

Novus Biologicals, part of Bio-Techne
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NBP3-18564-100ul
NBP3-18564-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13165

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WT1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a sythetic peptide for P19544. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for WT1 Antibody (CL13165)

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human fallopian tube and lymph node tissues using NBP3-18564 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.
Western Blot: WT1 Antibody (CL13165) [NBP3-18564]

Western Blot: WT1 Antibody (CL13165) [NBP3-18564]

Western Blot: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564]

Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564]

Immunocytochemistry/Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).
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Applications for WT1 Antibody (CL13165)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WT1

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Long Name

Wilms Tumor 1

Alternate Names

GUD, WAGR, WIT-2, WT33

Gene Symbol

WT1

Additional WT1 Products

Product Documents for WT1 Antibody (CL13165)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WT1 Antibody (CL13165)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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