WT1 Antibody (WT1/3477R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08966
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # WT1/3477R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa1-100) of human WT1 protein (exact sequence is proprietary) (Uniprot: P19544 )
Localization
Nucleus and cytoplasm. Shuttles between nucleus and cytoplasm.
Marker
Wilm's Tumor & Mesothelial Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07221).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for WT1 Antibody (WT1/3477R) - Azide and BSA Free
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) - Azide and BSA Free [NBP3-08966]
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) - Azide and BSA Free [NBP3-08966] - FFPE human kidney. PBS instead of primary antibody. Secondary antibody only negative control. Hematoxylin counterstain.Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) - Azide and BSA Free [NBP3-08966]
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) - Azide and BSA Free [NBP3-08966] - FFPE human kidney sections. Wilm's Tumor 1 labeled using WT1 (WT1/3477R) (1?g/ml). HIER: Tris/EDTA (pH9.0). HRP-Polymer. Hematoxylin counterstain.Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) - Azide and BSA Free [NBP3-08966]
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) - Azide and BSA Free [NBP3-08966] - FFPE human kidney. Secondary antibody only negative control. Hematoxylin counterstain.Applications for WT1 Antibody (WT1/3477R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: WT1
Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.
Long Name
Wilms Tumor 1
Alternate Names
GUD, WAGR, WIT-2, WT33
Gene Symbol
WT1
Additional WT1 Products
Product Documents for WT1 Antibody (WT1/3477R) - Azide and BSA Free
Product Specific Notices for WT1 Antibody (WT1/3477R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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