WT1 Antibody (WT1/857) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48216
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # WT1/857
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human WT1 protein (Uniprot: P19544)
Reactivity Notes
Weakly reacts with Mouse
Localization
Nuclear and Cytoplasmic
Specificity
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Marker
Wilm's Tumor & Mesothelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for WT1 Antibody (WT1/857) - IHC-Prediluted
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216]
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216] - Formalin-fixed, paraffin-embedded Rat Testis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216]
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216]
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216] - Formalin-fixed, paraffin-embedded HumanTestis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857)Applications for WT1 Antibody (WT1/857) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: WT1
Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.
Long Name
Wilms Tumor 1
Alternate Names
GUD, WAGR, WIT-2, WT33
Gene Symbol
WT1
Additional WT1 Products
Product Documents for WT1 Antibody (WT1/857) - IHC-Prediluted
Product Specific Notices for WT1 Antibody (WT1/857) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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