WWOX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97814

Novus Biologicals, part of Bio-Techne
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NBP2-97814-100ul
NBP2-97814-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WWOX Antibodies »

Product Summary for WWOX Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human WWOX fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for WWOX Antibody

Western Blot: WWOX Antibody [NBP2-97814]

Western Blot: WWOX Antibody [NBP2-97814]

Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa
Immunocytochemistry/ Immunofluorescence: WWOX Antibody [NBP2-97814]

Immunocytochemistry/ Immunofluorescence: WWOX Antibody [NBP2-97814]

Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-97814] - Immunofluorescence staining of WWOX in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human WWOX polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-97814]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-97814]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-97814] - Immunochemical staining of human WWOX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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Applications for WWOX Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WWOX

WW domain-containing oxidoreductase (WWOX) is a proapoptotic protein and a tumor suppressor gene. Because mutation or deletion in the coding region of WWOX is rarely found, it is speculated that the appearance of aberrant transcripts affects progression of many cancers. In addition, protein levels of WWOX, its Tyr33-phosphorylated form, and WOX2 are significantly down-regulated in the neurons of Alzheimer's hippocampi.

Long Name

WW Domain Containing Oxidoreductase

Alternate Names

FOR, FRA16D, SDR41C1, WOX1

Gene Symbol

WWOX

Additional WWOX Products

Product Documents for WWOX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WWOX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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