Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all WWP1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 200 and 250 of human WW Domain Containing E3 Ubiquitin Protein Ligase 1 using the numbering given in entry NP_008944.1 (GeneID 11059).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WWP1 Antibody

Western Blot: WWP1 Antibody [NBP1-49712]

Western Blot: WWP1 Antibody [NBP1-49712]

Western Blot: WWP1 Antibody [NBP1-49712] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WWP1 antibody NBP1-49712 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WWP1 was also immunoprecipitated by rabbit anti-WWP1 antibody NBP1-49713, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for WWP1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WWP1

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.

Long Name

WW Domain Containing E3 Ubiquitin Protein Ligase 1

Alternate Names

AIP5, HSDRP1, Tiul11

Gene Symbol

WWP1

Additional WWP1 Products

Product Documents for WWP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WWP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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