WWP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89179

Novus Biologicals, part of Bio-Techne
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NBP1-89179
NBP1-89179-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WWP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TIDLKQALLIHNRKLERVKEQLKLSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WWP1 Antibody

Immunocytochemistry/ Immunofluorescence: WWP1 Antibody [NBP1-89179]

Immunocytochemistry/ Immunofluorescence: WWP1 Antibody [NBP1-89179]

Immunocytochemistry/Immunofluorescence: WWP1 Antibody [NBP1-89179] - Staining of human cell line A-431 shows positivity in cytoplasm and golgi apparatus.
Immunohistochemistry-Paraffin: WWP1 Antibody [NBP1-89179]

Immunohistochemistry-Paraffin: WWP1 Antibody [NBP1-89179]

Immunohistochemistry-Paraffin: WWP1 Antibody [NBP1-89179] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells and moderate nuclear membrane staining in islet cells.
Immunocytochemistry/ Immunofluorescence: WWP1 Antibody [NBP1-89179]

Immunocytochemistry/ Immunofluorescence: WWP1 Antibody [NBP1-89179]

Immunocytochemistry/Immunofluorescence: WWP1 Antibody [NBP1-89179] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Applications for WWP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WWP1

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.

Long Name

WW Domain Containing E3 Ubiquitin Protein Ligase 1

Alternate Names

AIP5, HSDRP1, Tiul11

Gene Symbol

WWP1

Additional WWP1 Products

Product Documents for WWP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WWP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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