XAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56355

Novus Biologicals, part of Bio-Techne
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NB100-56355
NB100-56355SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all XAF1 Antibodies »

Product Specifications

Immunogen

Amino acids 201-218 (NQTSTMEKDVRPKTRSIN) and 301-317 (QLDLEKERYYKFKRFHF) of human XAF1, GenBank no. CAA68030.1, were used as immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XAF1 Antibody

Western Blot: XAF1 Antibody [NB100-56355]

Western Blot: XAF1 Antibody [NB100-56355]

Western Blot: XAF1 Antibody [NB100-56355] - analysis of XAF1 in human liver lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: XAF1 Antibody [NB100-56355]

Immunohistochemistry-Paraffin: XAF1 Antibody [NB100-56355]

Immunohistochemistry-Paraffin: XAF1 Antibody [NB100-56355] - XAF1 was detected in immersion fixed paraffin-embedded sections of human appendix using Rabbit Anti-Human XAF1 polyclonal Antibody (Catalog # NB100-56355) at 1:100 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells.

Applications for XAF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1-2 ug/ml
Application Notes
In human liver, a 34 kDa band should be observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XAF1

The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by virtue of inhibition of distinct caspases. The X-linked Inhibitor of Apoptosis, XIAP, is a key member of the IAP family of proteins. The caspase inhibiting activity of XIAP is negatively regulated by at least two XIAP-interacting proteins, XIAP-associated factor 1 (XAF1) and Smac/DIABLO. XAF1 binds to XIAP and antagonizes caspase inhibitory activities of XIAP. Expression of XAF1 triggers a redistribution of XIAP from the cytosol to the nucleus. XAF1 is ubiquitously expressed in normal tissues, but is present at low or undetectable levels in many different cancer cell lines.

Alternate Names

BIRC4-binding protein, BIRC4BPBIRC4 binding protein, HSXIAPAF1, XIAP associated factor 1, XIAPAF1XIAP-associated factor 1

Entrez Gene IDs

54739 (Human)

Gene Symbol

XAF1

Additional XAF1 Products

Product Documents for XAF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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