Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all XBP1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human XBP-1. The immunogen is located within the last 50 amino acids of XBP-1. Amino Acid Squence: LPAWRSSQRSTQKDPVPY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XBP1 Antibody - BSA Free

Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77252]

Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77252]

Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77252] - HepG2 cells with XBP-1 antibody at 2 ug/ml.
Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
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Applications for XBP1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Western Blot

0.5-2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-77252 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XBP1

X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Long Name

X-box Binding Protein 1

Alternate Names

HTF, TREB5, XBP2

Gene Symbol

XBP1

UniProt

P17861 (Human)

Additional XBP1 Products

Product Documents for XBP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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