XIAP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56183
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Recombinant BIR2 domain protein fragment of human XIAP was used as immunogen. The BIR2 domain used for immunogen corresponds to amino acids 163-230 of human XIAP (Deveraux et al, 1999).
Specificity
The antibody recognizes epitopes in the BIR2 domain of XIAP. Therefore it can recognize full-length XIAP and XIAP cleavage fragments containing the BIR2 domain. However, XIAP cleavage fragments may be biologically unstable, and therefore cleavage fragments may be difficult to detect.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for XIAP Antibody
Western Blot: XIAP Antibody [NB100-56183]
Western Blot: XIAP Antibody - Unpurified [NB100-56183] - Analysis of XIAP in various tumor cell lines using this antibody at 1:2000.Immunocytochemistry/ Immunofluorescence: XIAP Antibody [NB100-56183]
Immunocytochemistry/Immunofluorescence: XIAP Antibody [NB100-56183] - Immunocytochemistry for XIAP expression levels in IMR32 cells (Scale bar: 67 um). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-20499-7/figures/7) licensed under a CC-BY license.Immunohistochemistry-Paraffin: XIAP Antibody [NB100-56183]
Immunohistochemistry-Paraffin: XIAP Antibody - Unpurified [NB100-56183] - Human breast carcinoma using this antibody at 1:2000. A-A3, successively higher magnifications of the breast carcinoma tissue section. Hematoxylin-eosin counterstain.Applications for XIAP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 29391535)
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
1:50-1:200
Western Blot
1:1000-1:2000
Application Notes
Although not tested this antibody may be useful in Immunohistochemistry-Frozen.
Reviewed Applications
Read 3 reviews rated 4.3 using NB100-56183 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: XIAP
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
Additional XIAP Products
Product Documents for XIAP Antibody
Product Specific Notices for XIAP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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