XIAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56187

Novus Biologicals, part of Bio-Techne
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NB100-56187
NB100-56187SS
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all XIAP Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 241-260 (DAVSSDRNFPNSTNLPRNP) from the BIR3 domain of human XIAP was used as immunogen, GenBank no. NP_001158.2.

Specificity

The antibody recognizes epitopes in the BIR2 domain of XIAP. Therefore it can recognize full-length XIAP and XIAP cleavage fragments containing the BIR2 domain. However, XIAP cleavage fragments may be biologically unstable, and therefore cleavage fragments may be difficult to detect.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XIAP Antibody

Immunohistochemistry: XIAP Antibody [NB100-56187]

Immunohistochemistry: XIAP Antibody [NB100-56187]

Immunohistochemistry: XIAP Antibody [NB100-56187] - IHC analysis of XIAP in formalin-fixed, paraffin-embedded human small cell lung carcinoma at 1:2000. A, low magnification. A1, high magnification. Hematoxylin-eosin counterstain.

Applications for XIAP Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Neat whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XIAP

XIAP [human X-linked IAP, hILP (human IAP-like protein), MIHA, BIRC4) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including XIAP, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination (Morizane et al, 2005). Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. XIAP is highly characterized with respect to its structure and biochemical mechanisms, and has received interest as a therapeutic target (reviewed in Schimmer, 2006). Since XIAP blocks a substantial portion of the apoptosis pathway and is associated with chemoresistance in cancer cells, inhibiting XIAP has been a focus for potential therapeutics. Approaches have included antisense oligonucleotides and small molecule inhibitors. Small molecules that that target the BIR2 and BIR3 domains of XIAP are considered particularly attractive. This is because the BIR domains inhibit caspase activity, and it is thought that removing the inhibition should increase the cell's ability to undergo apoptosis as well as decrease its potential for chemoresistance. Full-length human XIAP is a 497 amino acid protein and migrates at approx. 53 kDa on SDS-PAGE.

Long Name

X-linked Inhibitor of Apoptosis

Alternate Names

BIRC4

Entrez Gene IDs

331 (Human)

Gene Symbol

XIAP

UniProt

P98170

Additional XIAP Products

Product Documents for XIAP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for XIAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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