XLF Antibody (OTI3D6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74876

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all XLF Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XLF Antibody (OTI3D6) - Azide and BSA Free

Western Blot: XLF Antibody (OTI3D6)Azide and BSA Free [NBP2-74876]

Western Blot: XLF Antibody (OTI3D6)Azide and BSA Free [NBP2-74876]

Western Blot: XLF Antibody (OTI3D6) - Azide and BSA Free [NBP2-74876] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XLF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XLF.
Immunocytochemistry/ Immunofluorescence: XLF Antibody (OTI3D6) - Azide and BSA Free [NBP2-74876]

Immunocytochemistry/ Immunofluorescence: XLF Antibody (OTI3D6) - Azide and BSA Free [NBP2-74876]

Immunocytochemistry/Immunofluorescence: XLF Antibody (OTI3D6) - Azide and BSA Free [NBP2-74876] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XLF.
Immunohistochemistry: XLF Antibody (OTI3D6) - Azide and BSA Free [NBP2-74876]

Immunohistochemistry: XLF Antibody (OTI3D6) - Azide and BSA Free [NBP2-74876]

Immunohistochemistry: XLF Antibody (OTI3D6) - Azide and BSA Free [NBP2-74876] - Staining of paraffin-embedded Human lymphoma tissue using anti-XLF mouse monoclonal antibody.
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Applications for XLF Antibody (OTI3D6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XLF

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Alternate Names

Cernunnos, non-homologous end-joining factor 1, nonhomologous end-joining factor 1, Protein cernunnos, XLFFLJ12610, XRCC4-like factor

Gene Symbol

NHEJ1

Additional XLF Products

Product Documents for XLF Antibody (OTI3D6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for XLF Antibody (OTI3D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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