XPB Antibody

Western Blot: XPB Antibody [NB100-61060] -

Western Blot: XPB Antibody [NB100-61060] - Effects of triptolide & histone depletion on condensation using mouse sperm.(A) Representative fluorescence images of chromatid assembly at steady state with mouse sperm nuclei in egg extracts (180 min after sperm nuclei addition) in the presence of indicated inhibitors. Triptolide or DMSO control was added at 25 min after nuclei addition. Triptolide was added at 50 μM. (B) Western blot for histone H3, CAP-E, & XPB in histone H4K12ac or IgG-depleted extracts in the presence of triptolide (TPL) or DMSO. Relative percentage of protein remaining after indicated treatments is shown.Figure 5—figure supplement 1—source data 1.Source data for Figure 5—figure supplement 1B.Source data for Figure 5—figure supplement 1B. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: XPB Antibody [NB100-61060] -

Western Blot: XPB Antibody [NB100-61060] - The TFIIH complex is required for the enrichment of condensins on chromosomes.(A) Left: Representative immunofluorescence images of Xenopus sperm nuclei incubated with Mock or CAP-E depleted extracts for 180 min. Chromatids were labeled with Hoechst & anti-XPB antibodies. Right: Quantification of fluorescence intensity of XPB on chromatin, normalized to Mock depleted condition. n = 24 structures for each condition. Error bars represent SD, & ‘NS’ indicates no statistically significant difference (p > 0.001). A.U., arbitrary units. Two biological replicates were performed, quantified structures are from a single experiment. (B) Western blot for indicated proteins that copurify with anti-XPB or IgG beads isolated from HSS extracts. IP, immunoprecipitate. (C) Western blot for indicated proteins that copurify with anti-CAP-E or Mock-treated beads isolated from HSS extracts. For condensin subunit detection, the blot was probed simultaneously with the indicated antibodies. Beads were coupled to either anti-CAP-E or pre-immune serum (“Mock”) & processed as indicated in the methods. IP, immunoprecipitate. (D) Representative immunofluorescence images of Xenopus sperm nuclei incubated with HSS extracts for 180 min. Chromatids were labeled with Hoechst & co-stained with anti-XPB & anti-CAP-E antibodies. Images of three representative individual chromatids are shown.Figure 3—figure supplement 1—source data 1.Source data for Figure 3—figure supplement 1B & C.Source data for Figure 3—figure supplement 1B & C. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: XPB Antibody [NB100-61060] -

Western Blot: XPB Antibody [NB100-61060] - The TFIIH complex is required for the enrichment of condensins on chromosomes.(A) Left: Representative immunofluorescence images of Xenopus sperm nuclei incubated with Mock or CAP-E depleted extracts for 180 min. Chromatids were labeled with Hoechst & anti-XPB antibodies. Right: Quantification of fluorescence intensity of XPB on chromatin, normalized to Mock depleted condition. n = 24 structures for each condition. Error bars represent SD, & ‘NS’ indicates no statistically significant difference (p > 0.001). A.U., arbitrary units. Two biological replicates were performed, quantified structures are from a single experiment. (B) Western blot for indicated proteins that copurify with anti-XPB or IgG beads isolated from HSS extracts. IP, immunoprecipitate. (C) Western blot for indicated proteins that copurify with anti-CAP-E or Mock-treated beads isolated from HSS extracts. For condensin subunit detection, the blot was probed simultaneously with the indicated antibodies. Beads were coupled to either anti-CAP-E or pre-immune serum (“Mock”) & processed as indicated in the methods. IP, immunoprecipitate. (D) Representative immunofluorescence images of Xenopus sperm nuclei incubated with HSS extracts for 180 min. Chromatids were labeled with Hoechst & co-stained with anti-XPB & anti-CAP-E antibodies. Images of three representative individual chromatids are shown.Figure 3—figure supplement 1—source data 1.Source data for Figure 3—figure supplement 1B & C.Source data for Figure 3—figure supplement 1B & C. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.