Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all XRCC1 Antibodies »

Product Summary for XRCC1 [p Ser485, p Thr488] Antibody

Immunogen

The immunogen recognized by this antibody maps to a region of surrounding residues Ser 485 and Thr 488 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515).

Modification

p Ser485, p Thr488

Specificity

NB100-541 is specific for human phospho-XRCC1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XRCC1 [p Ser485, p Thr488] Antibody

Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Whole cell lysate (50 ug) from asynchronously growing HeLa (H), 293T (T) and mouse NIH-3T3 (M) cells. Antibody used at 0.1 ug/ml. The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with
Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Sample: FFPE sections of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- Phospho-XRCC1-S485/T488 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Applications for XRCC1 [p Ser485, p Thr488] Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:5000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XRCC1

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Alternate Names

DNA repair protein XRCC1, RCC, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster

Entrez Gene IDs

7515 (Human)

Gene Symbol

XRCC1

UniProt

P18887 (Human)

Additional XRCC1 Products

Product Documents for XRCC1 [p Ser485, p Thr488] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for XRCC1 [p Ser485, p Thr488] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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