Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Drosophila, Hamster

Cited:

Human, Hamster, Insect - Drosophila

Applications

Validated:

ICC/IF (Negative), Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all XRCC3 Antibodies »

Product Specifications

Immunogen

A synthetic partial peptide of human XRCC3. [UniProt# O43542]

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 28743001). Hamster reactivity reported in scientific literature (PMID: 24116071).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XRCC3 Antibody - BSA Free

Western Blot: XRCC3 AntibodyBSA Free [NB100-165]

Western Blot: XRCC3 AntibodyBSA Free [NB100-165]

Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting [Yoshihara et all., 2004, EMBO J., 23 (3), 670-680]. hXRCC3 was detected by WB using XRCC3 antibody (NB100-165). The star indicates non-specific cross-reactivity of the antibody. Image provided by confirmed customer review.
Western Blot: XRCC3 AntibodyBSA Free [NB100-165]

Western Blot: XRCC3 AntibodyBSA Free [NB100-165]

Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from HeLa cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.

Applications for XRCC3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:5000
Application Notes
This XRCC3 antibody is useful for Western blot, where a band can be seen at 38 kDa. No cross-reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C, or RAD51D. The antibody is not applicable for Immunocytochemistry/Immunofluorescence.

Reviewed Applications

Read 1 review rated 5 using NB100-165 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: XRCC3

XRCC3 (X-ray repair cross-complementing group 3) belongs to RecA family and participates in HRR/homologous recombination repair pathway which is an "error free" DNA repair mechanism that utilizes information encoded by homologous sequence to repair double-strand breaks (DSBs), chromosomal fragmentation, translocations and deletions. XRCC3 is required for Rad51 assembly/stabilization and in HRR pathway, XRCC3 protein interacts with Rad51 protein for contributing to chromosome integrity maintainance, thereby preventing mutations which can lead to carcinogenesis. In addition to repairing DSBs, XRCC3 also plays a role repairing wide range of DNA damage arising from carcinogens. Moreover, XRCC3 can control mtDNA copy number under conditions of oxidative stress in the presence of RAD51 as well as RAD51C. It forms a part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3, and has been suggested to forms complex with FANCD2, BRCA2 and phosphorylated FANCG. XRCC3 also interacts with SWSAP1 and ZSWIM7 for executing homologous recombination repair and defective XRCC3 have been linked to susceptibility to breast cancer as well as cutaneous malignant melanoma type 6 (CMM6).

Alternate Names

CMM6, DNA repair protein XRCC3, X-ray repair complementing defective repair in Chinese hamster cells 3, X-ray repair cross-complementing protein 3

Gene Symbol

XRCC3

Additional XRCC3 Products

Product Documents for XRCC3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XRCC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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