YAP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46105
Key Product Details
Species Reactivity
Validated:
Canine
Predicted:
Human (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this YAP1 antibody maps to a synthectic peptide with the sequence of C-TYHSRDESTDS (NP_006097).
Epitope
internal region
Specificity
This antibody targets all isoforms of YAP1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for YAP1 Antibody
![Western Blot: YAP1 Antibody [NBP1-46105]](https://resources.bio-techne.com/images/products/YAP1-Antibody-Western-Blot-NBP1-46105-img0001.jpg "Western Blot: YAP1 Antibody [NBP1-46105]")
Western Blot: YAP1 Antibody [NBP1-46105]
Western Blot: YAP1 Antibody [NBP1-46105] - MDCK cell lysate (35 ug protein in RIPA buffer). YAP1 antibody dilution at 0.5 ug/mL, 1 hr incubation. Detected by chemiluminescence. Observed molecular weight is 65 kDa.Applications for YAP1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
0.5 - 2 ug/mL
Application Notes
WB: A customer has reported an approx. 70 kDa band observed in lysates of MDCK (calculated MW of 50.3 kDa band according to Dog XP_536601.2).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: YAP1
YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).
References
1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047
2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361
3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025
Long Name
Yes-associated Protein 1
Alternate Names
YAP2, YAP65, YKI, Yorkie Homolog
Entrez Gene IDs
10413 (Human)
Gene Symbol
YAP1
Additional YAP1 Products
Product Documents for YAP1 Antibody
Product Specific Notices for YAP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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