YB1 Antibody (4F12)
Novus Biologicals, part of Bio-Techne | Catalog # H00004904-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4F12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
YBX1 (NP_004550, 51 a.a. ~ 139 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKKVIATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYA
Specificity
YBX1 - nuclease sensitive element binding protein 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for YB1 Antibody (4F12)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01]](https://resources.bio-techne.com/images/products/YB1-Antibody-4F12-Immunocytochemistry-Immunofluorescence-H00004904-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01]")
Immunocytochemistry/ Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01]
Immunocytochemistry/Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01] - Analysis of monoclonal antibody to YBX1 on HeLa cell. Antibody concentration 20 ug/ml.![ELISA: YB1 Antibody (4F12) [H00004904-M01]](https://resources.bio-techne.com/images/products/YB1-Antibody-4F12-ELISA-H00004904-M01-img0006.jpg "ELISA: YB1 Antibody (4F12) [H00004904-M01]")
ELISA: YB1 Antibody (4F12) [H00004904-M01]
ELISA: YB1 Antibody (4F12) [H00004904-M01] - Detection limit for recombinant GST tagged YBX1 is approximately 0.3ng/ml as a capture antibody.Applications for YB1 Antibody (4F12)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: YB1
Alternate Names
BP-8, CBF-A, class II, Y box-binding protein I, CSDA2, CSDB, DBPB CCAAT-binding transcription factor I subunit A, EFI-A, Enhancer factor I subunit A, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP1 Y-box-binding protein 1, nuclease-sensitive element-binding protein 1, Y box binding protein 1, YB1 DNA-binding protein B, YB-1 Y-box transcription factor, YBX1
Entrez Gene IDs
4904 (Human)
Gene Symbol
YBX1
OMIM
154030 (Human)
UniProt
Additional YB1 Products
Product Documents for YB1 Antibody (4F12)
Product Specific Notices for YB1 Antibody (4F12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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