YB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89945
![Western Blot: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Western-Blot-NBP1-89945-img0013.jpg "Western Blot: YB1 Antibody [NBP1-89945]")
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0018.jpg "Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SGEKNEGSESAPEGQAQQRRPYRRRRFPPYYMRRPYGRRPQYSNPPVQGEVMEGADNQGAGEQGRPVRQNMYRGYRPRFRRGPPRQRQPREDGNEEDK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for YB1 Antibody
![Knockdown Validated: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Knockdown-Validated-NBP1-89945-img0019.jpg "Knockdown Validated: YB1 Antibody [NBP1-89945]")
Knockdown Validated: YB1 Antibody [NBP1-89945]
Knockdown Validated: YB1 Antibody [NBP1-89945] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human YB-1 Polyclonal Antibody (Catalog # NBP1-89945) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YB-1 at approximately 49 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.![Immunocytochemistry/ Immunofluorescence: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89945-img0011.jpg "Immunocytochemistry/ Immunofluorescence: YB1 Antibody [NBP1-89945]")
Immunocytochemistry/ Immunofluorescence: YB1 Antibody [NBP1-89945]
Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP1-89945] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.![Western Blot: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Western-Blot-NBP1-89945-img0008.jpg "Western Blot: YB1 Antibody [NBP1-89945]")
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0015.jpg "Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]")
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0016.jpg "Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]")
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]](https://resources.bio-techne.com/images/products/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0017.jpg "Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945]")
Applications for YB1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Knockdown Validated
0.4 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: YB1
Alternate Names
BP-8, CBF-A, class II, Y box-binding protein I, CSDA2, CSDB, DBPB CCAAT-binding transcription factor I subunit A, EFI-A, Enhancer factor I subunit A, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP1 Y-box-binding protein 1, nuclease-sensitive element-binding protein 1, Y box binding protein 1, YB1 DNA-binding protein B, YB-1 Y-box transcription factor, YBX1
Gene Symbol
YBX1
Additional YB1 Products
Product Documents for YB1 Antibody
Product Specific Notices for YB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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