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YTHDF3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94636

Novus Biologicals, part of Bio-Techne
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NBP2-94636-0.1ml
NBP2-94636-0.02ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human YTHDF3 (NP_689971.4). MSATSVDQRPKGQGNKVSVQNGSIHQKDAVNDDDFEPYLSSQTNQSNSYPPMSDPYMPSYYAPSIGFPYSLGEAAWSTAGDQPMPYLTTYGQMSNGEHHYIPDGVFSQPGALGNTPPFLGQHGFNFFPGNADFSTWGTSGSQGQSTQSSAYSSSYGYPPSSLGRAITDGQAGFGNDTLSKVPGISSIEQGMTGLKIGGDLTAAVTKTVGTALSSSGMTSIATNSVPPVSSAAPKPTSWAAIARKPAKPQPKLKPKGNVGIGGSAVPPPPIKHNMN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for YTHDF3 Antibody - BSA Free

Western Blot: YTHDF3 AntibodyBSA Free [NBP2-94636]

Western Blot: YTHDF3 AntibodyBSA Free [NBP2-94636]

Western Blot: YTHDF3 Antibody [NBP2-94636] - Western blot analysis of extracts of HepG2 cells, using YTHDF3 antibody (NBP2-94636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: YTHDF3 Antibody - BSA Free [NBP2-94636]

Immunohistochemistry-Paraffin: YTHDF3 Antibody - BSA Free [NBP2-94636]

Immunohistochemistry-Paraffin: YTHDF3 Antibody [NBP2-94636] - Immunohistochemistry of paraffin-embedded rat brain using YTHDF3 Rabbit pAb (NBP2-94636) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: YTHDF3 AntibodyBSA Free [NBP2-94636]

Western Blot: YTHDF3 AntibodyBSA Free [NBP2-94636]

Western Blot: YTHDF3 Antibody [NBP2-94636] - Western blot analysis of extracts of Mouse liver, using YTHDF3 antibody (NBP2-94636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for YTHDF3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: YTHDF3

Alternate Names

FLJ31657, YTH domain family 3, YTH domain family protein 3, YTH domain family, member 3

Gene Symbol

YTHDF3

Additional YTHDF3 Products

Product Documents for YTHDF3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for YTHDF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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