YY1 Antibody (6J6L1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15779
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6J6L1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY1 (P25490). PGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPH
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for YY1 Antibody (6J6L1)
![Western Blot: YY1 Antibody (6J6L1) [NBP3-15779]](https://resources.bio-techne.com/images/products/YY1-Antibody-6J6L1-Western-Blot-NBP3-15779-img0002.jpg "Western Blot: YY1 Antibody (6J6L1) [NBP3-15779]")
Western Blot: YY1 Antibody (6J6L1) [NBP3-15779]
Western Blot: YY1 Antibody (6J6L1) [NBP3-15779] - Western blot analysis of extracts of various cell lines, using YY1 antibody (NBP3-15779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunoprecipitation: YY1 Antibody (6J6L1) [NBP3-15779]](https://resources.bio-techne.com/images/products/YY1-Antibody-6J6L1-Immunoprecipitation-NBP3-15779-img0004.jpg "Immunoprecipitation: YY1 Antibody (6J6L1) [NBP3-15779]")
Immunoprecipitation: YY1 Antibody (6J6L1) [NBP3-15779]
Immunoprecipitation: YY1 Antibody (6J6L1) [NBP3-15779] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug YY1 antibody (NBP3-15779). Western blot was performed from the immunoprecipitate using YY1 antibody (NBP3-15779) at a dilition of 1:2000.
Western Blot: YY1 Antibody (6J6L1) [YY1] -
Western Blot: YY1 Antibody (6J6L1) [YY1] - Western blot analysis of various lysates using YY1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Applications for YY1 Antibody (6J6L1)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:1000 - 1:5000
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunoprecipitation
1:1000 - 1:5000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: YY1
Long Name
Yin-Yang-1
Alternate Names
DELTA, NF-E1, UCRBP
Gene Symbol
YY1
Additional YY1 Products
Product Documents for YY1 Antibody (6J6L1)
Product Specific Notices for YY1 Antibody (6J6L1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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