ZAK Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35439
Key Product Details
Species Reactivity
Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-312 of human ZAK (NP_598407.1).
Sequence:
EEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERE
Sequence:
EEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ZAK Antibody
Immunocytochemistry/ Immunofluorescence: ZAK Antibody [NBP3-35439] -
Immunocytochemistry/ Immunofluorescence: ZAK Antibody [NBP3-35439] - Immunofluorescence analysis of L929 cells using ZAK Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: ZAK Antibody [NBP3-35439] -
Western Blot: ZAK Antibody [NBP3-35439] - Western blot analysis of various lysates using ZAK Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Applications for ZAK Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ZAK
Alternate Names
AZK, EC 2.7.11, EC 2.7.11.25, HCCS-4, Human cervical cancer suppressor gene 4 protein, Leucine zipper- and sterile alpha motif-containing kinase, mitogen-activated protein kinase kinase kinase MLT, mixed lineage kinase 7, mixed lineage kinase with a leucine zipper and a sterile alpha motif, Mixed lineage kinase-related kinase, mixed lineage kinase-related kinase MRK-beta, MLK7, mlklak, MLK-like mitogen-activated protein triple kinase, MLK-related kinase, MLT, MLTK, MRKcervical cancer suppressor gene 4 protein, pk, protein kinase, sterile alpha motif and leucine zipper containing kinase AZK, Sterile alpha motif- and leucine zipper-containing kinase AZK
Gene Symbol
ZAK
Additional ZAK Products
Product Documents for ZAK Antibody
Product Specific Notices for ZAK Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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