ZBED3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76326

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ZBED3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ZBED3. The immunogen is located within the first 50 amino acids of ZBED3. Amino Acid Squence: GPAPTPTPPGRLGAPYS

Specificity

This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZBED3 Antibody - BSA Free

Western Blot: ZBED3 AntibodyBSA Free [NBP1-76326]

Western Blot: ZBED3 AntibodyBSA Free [NBP1-76326]

Western Blot: ZBED3 Antibody [NBP1-76326] - Western blot analysis of ZBED3 in A20 cell lysate with ZBED3 antibody at 1 ug/mL.

Applications for ZBED3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1-2 ug/ml
Application Notes
Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZBED3

Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.

Alternate Names

BED domain containing 3, MGC15435,2610005H11Rik, zinc finger, BED-type containing 3

Gene Symbol

ZBED3

UniProt

NP_115743 (Human)

Additional ZBED3 Products

Product Documents for ZBED3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZBED3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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