ZBTB4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76517

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ZBTB4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZBTB4. The immunogen is located within amino acids 790 - 840 of ZBTB4. Amino Acid Squence: AGTRPGDVKEEAPQEMQV

Specificity

At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZBTB4 Antibody - BSA Free

Western Blot: ZBTB4 AntibodyBSA Free [NBP1-76517]

Western Blot: ZBTB4 AntibodyBSA Free [NBP1-76517]

Western Blot: ZBTB4 Antibody [NBP1-76517] - SK-N-SH cell lysate with ZBTB4 antibody at 1 ug/mL.
Immunohistochemistry-Paraffin: ZBTB4 Antibody - BSA Free [NBP1-76517]

Immunohistochemistry-Paraffin: ZBTB4 Antibody - BSA Free [NBP1-76517]

Immunohistochemistry-Paraffin: ZBTB4 Antibody [NBP1-76517] - Human brain tissue with ZBTB4 antibody at 2.5 ug/mL.

Immunohistochemistry: ZBTB4 Antibody [NBP1-76517] -

Immunohistochemistry: ZBTB4 Antibody [NBP1-76517] - Human brain tissue with ZBTB4 antibody at 20 ug/mL.
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Applications for ZBTB4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZBTB4

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.

Alternate Names

KAISO-L1, zinc finger and BTB domain containing 4

Gene Symbol

ZBTB4

UniProt

EAW90185 (Human)

Additional ZBTB4 Products

Product Documents for ZBTB4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZBTB4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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