Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ZBTB5 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ZBTB6. The immunogen is located within the last 50 amino acids of ZBTB5. Amino Acid Squence: KRFSQSSHLYKHSKTT

Specificity

At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZBTB5 Antibody - BSA Free

Western Blot: ZBTB5 AntibodyBSA Free [NBP1-76518]

Western Blot: ZBTB5 AntibodyBSA Free [NBP1-76518]

Western Blot: ZBTB5 Antibody [NBP1-76518] - Mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 ug/mL.
ZBTB5 Antibody - BSA Free

Immunohistochemistry: ZBTB5 Antibody - BSA Free [NBP1-76518] -

Immunohistochemistry: ZBTB5 Antibody - BSA Free [NBP1-76518] - Immunohistochemistry of ZBTB5 in human brain tissue with ZBTB5 antibody at 2.5 ug/mL.
ZBTB5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZBTB5 Antibody - BSA Free [NBP1-76518] -

Immunocytochemistry/ Immunofluorescence: ZBTB5 Antibody - BSA Free [NBP1-76518] - Immunofluorescence of ZBTB5 in human brain tissue with ZBTB5 antibody at 20 ug/mL.

Applications for ZBTB5 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZBTB5

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.

Alternate Names

KIAA0354, zinc finger and BTB domain containing 5, zinc finger and BTB domain-containing protein 5

Gene Symbol

ZBTB5

UniProt

NP_055687 (Human)

Additional ZBTB5 Products

Product Documents for ZBTB5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZBTB5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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