ZC3H8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33980

Novus Biologicals, part of Bio-Techne
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NBP2-33980
NBP2-33980-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZC3H8 Antibodies »

Product Summary for ZC3H8 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NLKAGHKNGKQKKMKRKWPGPGNKGSNALLRNSGSQEEDGKPKEKQQHLSQAFINQHT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZC3H8 Antibody

Western Blot: ZC3H8 Antibody [NBP2-33980]

Western Blot: ZC3H8 Antibody [NBP2-33980]

Western Blot: ZC3H8 Antibody [NBP2-33980] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: MOLT-4
Immunocytochemistry/ Immunofluorescence: ZC3H8 Antibody [NBP2-33980]

Immunocytochemistry/ Immunofluorescence: ZC3H8 Antibody [NBP2-33980]

Immunocytochemistry/Immunofluorescence: ZC3H8 Antibody [NBP2-33980] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ZC3H8 Antibody [NBP2-33980]

Immunohistochemistry-Paraffin: ZC3H8 Antibody [NBP2-33980]

Immunohistochemistry-Paraffin: ZC3H8 Antibody [NBP2-33980] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Applications for ZC3H8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZC3H8

ZC3H8 acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds tothe 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Induces thymocyte apoptosis

Alternate Names

Fliz1, ZC3HDC8, zinc finger CCCH domain-containing protein 8, zinc finger CCCH type domain containing 8, zinc finger CCCH-type containing 8, zinc finger CCCH-type domain containing 8

Gene Symbol

ZC3H8

UniProt

Q8N5P1 (Human)

Additional ZC3H8 Products

Product Documents for ZC3H8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZC3H8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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