ZDHHC8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-57849
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QRDHPQLKTPPSK corresponding to internal region according to NP_037505.1.
Epitope
QRDHPQLKTPPSK
Specificity
This antibody is expected to recognise isoform 2 (NP_037505.1) only.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ZDHHC8 Antibody
![Western Blot: ZDHHC8 Antibody [NB100-57849]](https://resources.bio-techne.com/images/products/ZDHHC8-Antibody-Western-Blot-NB100-57849-img0002.jpg "Western Blot: ZDHHC8 Antibody [NB100-57849]")
Western Blot: ZDHHC8 Antibody [NB100-57849]
Western Blot: ZDHHC8 Antibody [NB100-57849] - (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for ZDHHC8 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:16000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 80-85 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 81.4 kDa band according to NP_037505.1).
An additional band of 48 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ZDHHC8
Alternate Names
DHHC8, EC 2.3.1, EC 2.3.1.-, KIAA1292DHHC-8, membrane-associated DHHC8 zinc finger protein, probable palmitoyltransferase ZDHHC8, ZDHHCL1, Zinc finger DHHC domain-containing protein 8, Zinc finger protein 378, zinc finger, DHHC-type containing 8, ZNF378zinc finger, DHHC domain like containing 1
Gene Symbol
ZDHHC8
UniProt
Additional ZDHHC8 Products
Product Documents for ZDHHC8 Antibody
Product Specific Notices for ZDHHC8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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