Western Blot: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 expression was assayed in lung adenocarcinoma cells after transfectionwith miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells. Image submitted by verified customer review. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.

Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining. Image from the standard format of this antibody.

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.

Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining. Image from the standard format of this antibody.

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on Jurkat with ZEB1 Antibody (2A8A6) NBP2-23484 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B). Image from the standard format of this antibody.

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on U-87 cells with ZEB1 [2A8A6] Antibody NBP2-23484AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on with A549 cells with ZEB1 Antibody NBP2-23484PE and a a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.

CyTOF-ready: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - Analysis of ZEB1 antibody on Human tissue. ZEB1 expression in the epithelial layers (2 outer sides) and mesenchymal cells (middle area). Image from verified customer review.

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Applications for ZEB1 Antibody (2A8A6) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1: 500 - 1:2000. Use reported by customer review and in scientific literature (PMID 29383119)

Application Notes

This antibody has had weak results in Western blot.

Reviewed Applications

Read 1 review rated 4 using NBP2-81015 in the following applications:

CyTOF (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (2A8A6) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ZEB1 Antibody (2A8A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

ZEB1 Antibody (2A8A6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81015

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

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Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for ZEB1 Antibody (2A8A6) - Azide and BSA Free

Western Blot: ZEB1 Antibody (2A8A6)Azide and BSA Free [NBP2-81015]

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]

Applications for ZEB1 Antibody (2A8A6) - Azide and BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: ZEB1

Long Name

Alternate Names

Gene Symbol

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (2A8A6) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for ZEB1 Antibody (2A8A6) - Azide and BSA Free