ZEB1 Antibody (2A8H3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37329

Novus Biologicals, part of Bio-Techne
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NBP2-37329
NBP2-37329-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2A8H3

Format

BSA Free

Concentration

1 mg/ml

View all ZEB1 Antibodies »

Product Summary for ZEB1 Antibody (2A8H3) - BSA Free

Immunogen

Purified recombinant fragment of human ZEB1 (AA: 967-1108) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZEB1 Antibody (2A8H3) - BSA Free

Western Blot: ZEB1 Antibody (2A8H3)BSA Free [NBP2-37329]

Western Blot: ZEB1 Antibody (2A8H3)BSA Free [NBP2-37329]

Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8H3) - BSA Free [NBP2-37329]

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8H3) - BSA Free [NBP2-37329]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: ZEB1 Antibody (2A8H3) - BSA Free [NBP2-37329]

Immunohistochemistry: ZEB1 Antibody (2A8H3) - BSA Free [NBP2-37329]

Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.
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Applications for ZEB1 Antibody (2A8H3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (2A8H3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZEB1 Antibody (2A8H3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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