In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?

Name: ZEB1 Antibody (4C4)
Brand: Novus Biologicals
SKU: H00006935-M01

ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

ZEB1 Antibody (4C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006935-M01

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

ZEB1 (NP_110378, 801 a.a. ~ 900 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTE

Specificity

transcription factor 8 (represses interleukin 2 expression)

Marker

Mesenchymal Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ZEB1 Antibody (4C4)

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - U-2 OS cells were stained with ZEB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on U-2 OS cell. Antibody concentration 10 ug/ml.

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