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ZEB1 Antibody (CL0151) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43907

Novus Biologicals, part of Bio-Techne
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NBP3-43907

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0151

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P37275, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ZEB1 Antibody (CL0151) - Azide and BSA Free

ZEB1 Antibody (CL0151) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]

Staining in A549 cell line with Anti-ZEB1 monoclonal antibody) staining in green. Microtubule probes are visualized in red (where available).
ZEB1 Antibody (CL0151) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]

Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]

Analysis in human endometrium and liver tissues using NBP3-43907 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.
ZEB1 Antibody (CL0151) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]

Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody) staining in green. Microtubule probes are visualized in red (where available).

Applications for ZEB1 Antibody (CL0151) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (CL0151) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZEB1 Antibody (CL0151) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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