ZEB1 Antibody (HL2245) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25767
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation, Independent Antibodies
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2245
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human ZEB1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZEB1 Antibody (HL2245) - Azide and BSA Free
Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] -
Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] -
Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] -
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Applications for ZEB1 Antibody (HL2245) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Gene Symbol
ZEB1
Additional ZEB1 Products
Product Documents for ZEB1 Antibody (HL2245) - Azide and BSA Free
Product Specific Notices for ZEB1 Antibody (HL2245) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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