ZEB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68930

Novus Biologicals, part of Bio-Techne
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NBP1-68930
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all ZEB1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to ZEB1 (zinc finger E-box binding homeobox 1) The peptide sequence was selected from the N terminal of ZEB1. Peptide sequence NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT. The peptide sequence for this immunogen was taken from within the described region.

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for ZEB1 Antibody

Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: ZEB1.
Immunohistochemistry: ZEB1 Antibody [NBP1-68930]

Immunohistochemistry: ZEB1 Antibody [NBP1-68930]

Immunohistochemistry: ZEB1 Antibody [NBP1-68930] - Human Capan1 cells (Pancreatic cancer cell line) Primary Antibody Dilution: 1 : 300 Secondary Antibody: Anti-rabbit-AlexaFluor-488 Secondary Antibody Dilution: 1 : 200Color/Signal.
Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930] - Lane 1: Control, Lane 2: ZEB1 morphants. Upper panel: ZEB1 blot; Bottom panel: tubulin blot
View 1 more images

Applications for ZEB1 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Reviewed Applications

Read 1 review rated 3 using NBP1-68930 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

UniProt

P37275

Additional ZEB1 Products

Product Documents for ZEB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZEB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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